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To test this prediction, we reintroduced chromosomal Lon expression under the Charge of a constitutive promoter inside our assortment strain, and recurring deep mutational scanning in biological triplicate (Supplementary file 2). We refer to The 2 regimes as +Lon and –Lon variety. the standard of +Lon assortment was comparable to that of –Lon variety (Figure 2—figure dietary supplement one, determine 2—figure supplement two). per our hypothesis, the distribution of collection coefficients shifted toward far more adverse values from the +Lon selection, depleting favourable range coefficients and enriching for damaging or null coefficients (Figure 2A).
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